Hydrocarbons are usually called hydrocarbons. Organic compounds composed of two elements of carbon and hydrogen are called hydrocarbons. The main components of beeswax are compounds synthesized from high-grade fatty acids and monohydric alcohols, fatty acids and hydrocarbons. Compound.
This standard applies to beeswax with a hydrocarbon content of 14%-40%
Principle: SThe sample is dissolved in petroleum ether and passed through a silica gel column. The lipids, acids and other polar substances in beeswax are adsorbed by silica gel, and non-polar hydrocarbons are separated out, and then quantified by weight method
Reagents:Silica gel chromatography uses 100-200 mesh. Before use, it needs to be activated in an oven at 125° for 5-6 hours, then take it out and place it in a desiccator for later use.Petroleum ether: analytically pure, boiling range 30-60 and 60-90 (petroleum ether is redistilled before use)
Instrument: chromatography column, length 30-40cm, inner diameter 2cm
Operate and calculate according to SN/T 0621-1996 standard